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M9480494.TXT
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1994-08-20
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Document 0494
DOCN M9480494
TI Specificity and sequence requirements for interactions between various
retroviral Gag proteins.
DT 9410
AU Franke EK; Yuan HE; Bossolt KL; Goff SP; Luban J; Department of
Medicine, Columbia University, College of; Physicians and Surgeons, New
York, New York 10032.
SO J Virol. 1994 Aug;68(8):5300-5. Unique Identifier : AIDSLINE
MED/94309202
AB We previously established a genetic assay for retroviral Gag polyprotein
multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S.
P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to
demonstrate homomeric interactions between Gag polyproteins encoded by
six different retroviruses. Of the Gag polyproteins tested, only those
encoded by closely related retroviruses formed heteromultimers. To
determine the primary sequence requirements for human immunodeficiency
virus type 1 Gag polyprotein multimerization, we studied the effects on
multimerization of deletion and linker insertion mutations. Sequences
necessary for this process were located between the C-terminal one-third
of the capsid domain and the C terminus of the nucleocapsid domain.
DE Animal Base Sequence DNA, Viral Gene Products,
gag/GENETICS/*METABOLISM Genetic Techniques Human Molecular Sequence
Data Mutagenesis, Insertional Retroviridae/GENETICS/*METABOLISM
Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).